RNA

Part:BBa_K3086003:Design

Designed by: Nikila Ojili   Group: iGEM19_Florida   (2019-10-18)


msr +msd(rpoB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to insert the rpoB sequence into msd using ligation independent cloning. The msDNA (containing the rpoB target sequence) had to be designed such that the it would be homologous to the Okazaki fragment during DNA replication.


Source

PFF745 plasmid from lab of Prof. Tim Lu (iGEM registry Bba_K1681000)

References